膜杰作
Star Staining
描述（Description）
The Human TIGIT (Luc) Jurkat Reporter Cell was engineered to not only express the NFAT response element driving luciferase expressing systems, but also express the receptor full length human TIGIT (Gene ID: 201633), which can use to evaluate the potency of TIGIT blockade. When co-cultured with target cells expressing human CD155, the TIGIT/CD155 interaction inhibits TCR signaling and NFAT-mediated luminescence. Blocking the TIGIT/CD155 interaction by either anti-TIGIT or anti-CD155 antibodies releases the inhibitory signal and results in TCR activation and NFAT-mediated luminescence.
应用说明（Application）
Screen for anti-human TIGIT or anti-human CD155 antibody.
生长特性（Growth Properties）
Suspension
筛选标记（Selection Marker）
Puromycin (5 μg/mL) + Hygromycin (20 μg/mL)
培养基（Culture Medium）
RPMI-1640 + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Receptor Assay
Expression analysis of human TIGIT on Human TIGIT (Luc) Jurkat Reporter Cell by FACS.
Human TIGIT (Luc) Jurkat Reporter Cell or negative control cell were stained with PE-labeled anti-human TIGIT antibody.
Protocol
Application
Blocking activity of anti-human TIGIT antibody.
This reporter cell was incubated with serial dilutions of antibodies in the presence of target cells expressing human CD155. The EC50 of anti-human TIGIT antibody was approximately 2.05 μg/mL.
Protocol
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背景（Background）
T cell immunoglobulin and ITIM domain (TIGIT) is an immune checkpoint that has been considered as a target in cancer immunotherapy and belongs to the family of poliovirus receptors (PVRs) that competes against CD226 (DNAM-1), which is the co-stimulatory receptor of T cells and NK cells, by binding to CD112 and CD155 (PVR) that are expressed on antigen-presenting cells (APCs) and various non-hematopoietic cell types, such as tumor cells. The TIGIT/PVR axis imparts a co-inhibitory effect on both T and NK cells that lead to their exhaustion, in turn triggering a negative signal that suppresses their activities, resulting in tumor-immune evasion.
License Disclosure
This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
