Human Interferon-γ (IFN-γ) ELISA Assay Pair

背景（Background）

Cytokine storm syndromes is an activation cascade of pro-inflammatory cytokines, such as TNF-α, IL-1, IL-6, IL-12, IFN-α, IFN-β, IFN-γ, MCP-1 and IL-8, due to unregulated host immune response system to different stimuli such as infections, malignancy, rheumatoid disorders, drugs, and so on. Interferon-γ (IFN-γ), one of the four subclasses ofinterferons, and is a multipotent protein, acting on many cell types by inducing or inhibiting many cellular functions through direct effects on gene expression. This ELISA assay pair contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IFN-γ.

应用说明（Application）

The pair is developed for quantitative detection of IFN-γ in human serum and cell culture supernates.

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储（Storage）

Unopened kit should be stored at 2°C -8°C upon receiving.

Find the expiration date on the outside packaging and do not use reagents past their expiration date.

The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

原理（Assay Principles）

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Human IFN-γ. The kit consists of Human IFN-γ Capture Antibody and Human IFN-γ Standard and Human IFN-γ Detection Antibody and Streptavidin-HRP.

Your experiment will include 5 simple steps:

a) Coat the plate with Human IFN-γ Capture Antibody.

b) Add your molecule of interest to the tests.

c) Add Human IFN-γ Detection Antibody.

d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis.