分子别名(Synonym)
Cadherin-17,CDH17,HPT-1,LI-cadherin
表达区间及表达系统(Source)
Biotinylated Human Cadherin-17, His,Avitag (CA7-H52E6) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Met 787 (Accession # Q12864-1).
Predicted N-terminus: Gln 23
Request for sequence
蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 88.6 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度(Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Biotinylated Human Cadherin-17, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human Cadherin-17, His,Avitag (Cat. No. CA7-H52E6) is more than 90% and the molecular weight of this protein is around 95-115 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
Immobilized Human Anti-Cadherin 17 at 0.1% can bind Biotinylated Human Cadherin-17, His,Avitag (Cat. No. CA7-H52E6) with a linear range of 0.2-16 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human Cadherin-17, His,Avitag (Cat. No. CA7-H52E6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind various dilution ratio of Human Anti-Cadherin 17 (Routinely tested).
Protocol
背景(Background)
Cadherin-17, also known as liver-intestine (LI) Cadherin, belongs to the cadherin family of calcium-dependent cell adhesion molecules. In vivo studies showed CDH17 knockout resulted in apoptotic PC tumor death through activating caspase-3 activity. Taken together, CDH17 functions as an oncogenic molecule critical to PC growth by regulating tumor apoptosis signaling pathways and CDH17 could be targeted to develop an anti-PC therapeutic approach.