分子别名(Synonym)
Rep 68
表达区间及表达系统(Source)
Adeno-associated virus 2 (isolate Srivastava/1982) (AAV-2) Rep 68, His Tag (RE8-A5243) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Leu 536 (Accession # P03132-1 ).
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蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 62.8 kDa. The protein migrates as 75-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Adeno-associated virus 2 (isolate Srivastava/1982) (AAV-2) Rep 68, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性(Bioactivity)-ELISA
Immobilized Adeno-associated virus 2 (isolate Srivastava/1982) (AAV-2) Rep 68, His Tag (Cat. No. RE8-A5243) at 1 μg/mL (100 μL/well) can bind Monoclonal Mouse anti‑Adeno‑Associated Virus AAV2 Rep68 Antibody with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
背景(Background)
Adeno-associated virus (AAV) is a single-stranded D virus, and the current scientific consensus is that it does not cause any human disease. It consists of a protein capsid and a single-stranded D genome of 4.7 kb in length, with two 'T' terminal inverted repeats (ITRs) at each end of the AAV genome. The rep genes in the AAV genome encode four proteins associated with viral replication, Rep78, Rep68, Rep52, and Rep40.