膜杰作
Star Staining
分子别名（Synonym）
CLEC12A,MICL,CLL-1,CLL1,DCAL2,DCAL-2,CD371
表达区间及表达系统（Source）
APC-Labeled Human CLEC12A, His Tag (CLA-HA248) is produced via conjugation of APC to Human CLEC12A, His Tag with a new generation site-specific technology under optimal conditions with a proprietary technology. Human CLEC12A, His Tag is expressed from human 293 cells (HEK293). It contains AA His 65 - Ala 265 (Accession # NP_612210.4).
Predicted N-terminus: Gly
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus
The protein has a calculated MW of 27.3 kDa.
偶联（Conjugate）
APC
Excitation Wavelength: 640 nm
Emission Wavelength: 661 nm
应用说明（Application）
Please note that this product is NOT compatible to streptavidin detection system.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
活性（Bioactivity）-FACS
5e5 of anti-CLEC12A / CLL-1 CAR-293 cells were stained with 100 μL of 1:25 dilution (4 μL stock solution in 100 μL FACS buffer) of APC-Labeled Human CLEC12A, His Tag (Cat. No. CLA-HA248) and negative control protein respectively. APC signal was used to evaluate the binding activity (QC tested).
Protocol
背景（Background）
CLEC12A (C-type lectin domain family 12 member A) is also known as CLL1, DCAL2, MICL. Clec12a is an inhibitory receptor for uric acid crystals that regulates inflammation in response to cell death. Cell surface receptor that modulates signaling cascades and mediates tyrosine phosphorylation of target MAP kinases. Evidence of distinct disease propagating stem cells in myelodysplastic syndrome (MDS) has emerged in recent years. The role of CLEC12A in MDS, however, remains to be elucidated. Furthermore, CLEC12A has been proposed as a promising marker of leukaemic stem cells in AML.
