分子别名(Synonym)
BTLA,CD272
表达区间及表达系统(Source)
Biotinylated Human BTLA (31-150) (M105V, G138S, V148M), Fc,Avitag (BTA-H82F5) is expressed from human 293 cells (HEK293). It contains AA Lys 31 - Ser 150 (Accession # AAP44003.1 (M105V,G138S,V148M)).
Predicted N-terminus: Lys 31
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蛋白结构(Molecular Characterization)
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 41.9 kDa. The protein migrates as 53-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度(Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Biotinylated Human BTLA (31-150) (M105V, G138S, V148M), Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human BTLA (31-150) (M105V, G138S, V148M), Fc,Avitag (Cat. No. BTA-H82F5) is more than 85% and the molecular weight of this protein is around 108-128 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
Immobilized Human HVEM, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. HVM-H5255) at 5 μg/mL (100 μL/well) can bind Biotinylated Human BTLA (31-150) (M105V, G138S, V148M), Fc,Avitag (Cat. No. BTA-H82F5) with a linear range of 0.001-0.313 μg/mL (QC tested).
Protocol
Immobilized Biotinylated Human BTLA (31-150) (M105V, G138S, V148M), Fc,Avitag (Cat. No. BTA-H82F5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human HVEM, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. HVM-H5255) with a linear range of 1-20 ng/mL (Routinely tested).
Protocol
背景(Background)
B- and T-lymphocyte attenuator (BTLA) is also known as B- and T-lymphocyte-associated protein, CD antigen CD272. BTLA contains one Ig-like V-type (immunoglobulin-like) domain. As a lymphocyte inhibitory receptor, BTLA / CD272 inhibits lymphocytes during immune response. BTLA / CD272 can interact with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, and interact with TNFRSF14/HVEM.