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 >  Protein>LYPD1 >LY1-H5254

Human LYPD1 / PHTS protein, Fc Tag (MALS verified)

分子别名(Synonym)

LYPD1,Ly6/PLAUR domain-containing protein 1,FLJ41033,LY6/PLAUR domain containing 1,LYPDC1,PHTS,Putative HeLa tumor suppressor

表达区间及表达系统(Source)

Human LYPD1, Fc Tag (LY1-H5254) is expressed from human 293 cells (HEK293). It contains AA Leu 21 - Gly 115 (Accession # Q8N2G4-1).

Predicted N-terminus: Leu 21

Request for sequence

蛋白结构(Molecular Characterization)

LYPD1 Structure

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 36.8 kDa . The protein migrates as 43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

LYPD1 SDS-PAGE

Human LYPD1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

LYPD1 SEC-MALS

The purity of Human LYPD1, Fc Tag (Cat. No. LY1-H5254) is more than 85% and the molecular weight of this protein is around 142-172 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

LYPD1 ELISA

Immobilized Human LYPD1, Fc Tag (Cat. No. LY1-H5254) at 1 μg/mL (100 μL/well) can bind Anti-LYPD1 antibody with a linear range of 0.02-0.625 μg/mL (QC tested).

Protocol

 

背景(Background)

Believed to act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity. In vitro increases receptor desensitization and decreases affinity for ACh of alpha-4:beta-2-containing nAChRs. May play a role in the intracellular trafficking of alpha-4:beta-2 and alpha-7-containing nAChRs and may inhibit their expression at the cell surface. May be involved in the control of anxiety.

 

前沿进展

 
 
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