膜杰作
Star Staining
分子别名（Synonym）
CRISPR-associated endonuclease Cas12a, CRISPR-associated endonuclease Cpf1, cas12a, Cpf1
表达区间及表达系统（Source）
GENPower™ NLS-Cas12a Nuclease (CAA-L5140) is expressed from E. coli cells. It contains AA Ser 2 - His 1228 (Accession # A0A5S8WF58).
Predicted N-terminus: Met
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 147.6 kDa. The protein migrates as 135-145 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE). This protein has two NLS, which leads to transport to the nucleus efficiently. It is ideal for in vitro and in vivo reactions.
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method.
无菌（Sterility）
The sterility testing was performed by membrane filtration method.
浓度（Concentration）
10μg/μL
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 20 mM NaAC,500 mM NaCl,0.1 mM EDTA,0.1 mM TCEP,pH 6.0 with glycerol as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -20°C or lower upon receipt;
- -20°C for 3 months under sterile conditions.
电泳（SDS-PAGE）
GENPower™ NLS-Cas12a Nuclease on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of GENPower™ NLS-Cas12a Nuclease (Cat. No. CAA-L5140) is more than 90% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.
Report
活性（Bioactivity）
Measured by its ability to cleave a targeted DNA substrate. Cas12a achieves >90% substrate cleavage (QC tested).
背景（Background）
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated endonuclease from Lachnospiraceae bacterium，Cas12a, also known as Cpf1, is a 1200-1500 amino-acids long monomeric protein that belongs to the CRISPR/Cas system , an adaptive immune system of prokaryotes that has now become a powerful tool for genome editing. CRISPR/Cas12a belongs the class II (type 5) of the CRISPR/Cas system that is defined by a single-subunit effector. Cas12a has recently emerged as an alternative for Cas9, due to its distinct features such as the ability to target T-rich motifs, no need for trans-activating crRNA, inducing a staggered double-strand break and potential for both RNA processing and DNA nuclease activity. In addition, Cas12a is able to process more structured pre-CRISPR/RNA(crRNA) molecules into mature crRNAs which allows the possibility to use both mature or pre-crRNA for genome editing purposes. All these features make the CRISPR/Cas12a system a valuable genome-engineering tool. CRISPR-Cas12a has been successfully used to edit genomes in many mammalians cells and plants.
