Human HTT, MBP,His Tag (HTT-H51M5) is expressed from E.coli cells. It contains AA Met 1 - Glu 82 (Accession # P42858-1 ).
Predicted N-terminus: Met
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This protein carries a MBP tag at the N-terminus and a polyhistidine tag at the C-terminus
The protein has a calculated MW of 52.7 kDa. The protein migrates as 60-63 kDa under reducing (R) condition (SDS-PAGE).
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human HTT, MBP,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human HTT, MBP,His Tag (Cat. No. HTT-H51M5) is more than 85% and the molecular weight of this protein is around 62-80 kDa verified by SEC-MALS.
Huntingtin is the protein coded for by the HTT gene. Autosomal dominant mutation in the Huntingtin (Htt) protein is the cause of Huntington’s Disease (HD). In HD, the polyglutamine (polyQ) domain in the N-terminal sequenceof the protein is expanded beyond a threshold of 36 glutamines. mutant polyQ expansion strongly correlates in an inverse manner to disease age of onset.