膜杰作
Star Staining
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Aneuro
ComboX
ActiveMax
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Star Ribbon
抗体来源（Source）
Monoclonal Anti-His Tag Antibody,Mouse IgG1 (AY63) antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with His Tag Protein.
亚型（Isotype）
Mouse IgG1 | Mouse Kappa
特异性（Specificity）
This product is a specific antibody specifically reacts with His tag protein, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
应用（Application）
ELISA
纯度（Purity）
>95% as determined by SDS-PAGE.
内毒素（Endotoxin）
Less than 1.0 EU per mg by the LAL method.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS,pH 7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Monoclonal Anti-His Tag Antibody, Mouse IgG1 (AY63) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized Human IL-4 R alpha Protein, His Tag (Cat. No. ILR-H5221) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-His Tag Antibody, Mouse IgG1 (AY63) (Cat. No. HIS-AY63) with a linear range of 0.20-3.13 ng/mL (QC tested)
Protocol
背景（Background）
His tag is a very small molecular weight tag, usually composed of 6-10 histidine (His). It is one of the commonly used tags for protein purification and detection. Due to its small molecular weight, fusion into the target protein has almost no effect on the structure and characteristics of the protein. Anti-his tag antibody can accurately detect, locate and purify His tag fusion protein, so as to provide convenience for the vast number of researchers.
