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Human Transforming growth factor-alpha/TGF-alpha Protein, His Tag

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分子别名(Synonym)

Transforming growth factor-alpha

表达区间及表达系统(Source)

Human Transforming growth factor-alpha/TGF-alpha, His Tag (TGA-H5141) is expressed from human 293 cells (HEK293). It contains AA Val 40 - Ala 89 (Accession # P01135-1).

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

TGF-alpha Structure

This protein carries a polyhistidine tag at the N-terminus

The protein has a calculated MW of 7.4 kDa. The protein migrates as 11 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

TGF-alpha SDS-PAGE

Human Transforming growth factor-alpha/TGF-alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

 

活性(Bioactivity)-ELISA

TGF-alpha ELISA

Immobilized Human Transforming growth factor-alpha/TGF-alpha, His Tag (Cat. No. TGA-H5141) at 5 μg/mL (100 μL/well) can bind Human EGF R, Fc Tag (Cat. No. EGR-H5252) with a linear range of 0.2-2 ng/mL (QC tested).

Protocol

TGF-alpha ELISA

Immobilized Human Transforming growth factor-alpha/TGF-alpha, His Tag (Cat. No. TGA-H5141) at 5 μg/mL (100 μL/well) can bind Biotinylated Human EGF R, Fc,Avitag (Cat. No. EGR-H82F8) with a linear range of 0.3-10 ng/mL (Routinely tested).

Protocol

 

背景(Background)

TGF-alpha is a normal physiological ligand for the EGF receptor. TGF-α and the EGF receptor are expressed in many developing and adult tissues, where TGF-α has been implicated in cell migration, growth, and differentiation.

 

前沿进展

 
 
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TGF-alpha靶点信息
英文全称:Transforming growth factor alpha
中文全称:转化生长因子α
种类:
上市药物数量:0详情
临床药物数量:1详情
最高研发阶段:临床二期
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