登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>Pre-Fusion glycoprotein F0 >RSF-V82E7

Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0 Protein, His,Avitag™(MALS verified)

热销产品推荐:

利用生物素-链霉亲和素系统的高特异性和高灵敏度的结合特性,可将生物素化蛋白与链霉亲和素相关产品联合应用于各类实验,与非生物素体系相比,检测速度更快、结合能力更强、非特异背景更低。

表达区间及表达系统(Source)

Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0, His,Avitag (RSF-V82E7) is expressed from human 293 cells (HEK293).

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

The protein has a calculated MW of 57.8 kDa. The protein migrates as 65-67 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Pre-Fusion glycoprotein F0 SDS-PAGE

Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Pre-Fusion glycoprotein F0 SEC-MALS

The purity of Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0, His,Avitag (Cat. No. RSF-V82E7) is more than 85% and the molecular weight of this protein is around 185-225 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Pre-Fusion glycoprotein F0 ELISA

Immobilized Anti-Fusion glycoprotein F0 Antibody, Human IgG1 (D25) at 5 μg/mL (100 μL/well) can bind Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0, His,Avitag (Cat. No. RSF-V82E7) with a linear range of 0.1-8 ng/mL (QC tested).

Protocol

Pre-Fusion glycoprotein F0 ELISA

Immobilized Anti-Fusion glycoprotein F0 Antibody, Mouse IgG2a (101F) at 5 μg/mL (100 μL/well) can bind Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0, His,Avitag (Cat. No. RSF-V82E7) with a linear range of 0.2-16 ng/mL (QC tested).

Protocol

Pre-Fusion glycoprotein F0 ELISA

Immobilized Recombinant Mouse Anti-RSV F Antibody (5C4) at 5 μg/mL (100 μL/well) can bind Biotinylated Human respiratory syncytial virus A (strain A2) Pre-F0, His,Avitag (Cat. No. RSF-V82E7) with a linear range of 0.2-16 ng/mL (QC tested).

Protocol

 

背景(Background)

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. The RSV fusion glycoprotein (RSV F) is the principal target of RSV neutralizing antibodies in human sera. The RSV F is a type I viral fusion protein synthesized as inactive, single-chain polypeptides that assemble into trimers. RSV F fuses the viral and host cell membranes by irreversible protein refolding from the labile prefusion conformation to the stable post-fusion conformation.

 

前沿进展

 
 
货号/价格
产品推荐
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定