膜杰作
Star Staining
产品描述（Product Details）
Laminin-121 is one of the α1 chain containing laminins. The α1 chain is detected at the 2-cell stage of the pre-implantation mouse embryo and is subsequently expressed mainly in many epithelial tissues during organogenesis. Laminin-121 showed the highest affinity to α6β1 and α7β1 integrins and furthermore, laminin-121 most effectively supported neurite outgrowth.
产品参数（Key parameter）
| Purity (SDS PAGE) | > 90% |
| Mycoplasma Test | Negative |
| Sterility Test | Negative |
| Endotoxin Test | < 0.01 EU per μg |
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
活性（Bioactivity）-ELISA
Immobilized Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) at 5 μg/mL (100 μL/well) can bind Human Laminin 121 Protein, premium grade (Cat. No. LA1-H5261) with a linear range of 0.5-62.5 ng/mL (Routinely tested).
Protocol
Immobilized Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) at 5 μg/mL (100 μL/well) can bind Human Laminin 121 Protein, premium grade (Cat. No. LA1-H5261) with a linear range of 0.2-8 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human Laminin 121 Protein, premium grade (Cat. No. LA1-H5261) on Protein A Biosensor, can bind Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) with an affinity constant of 6.23 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
Loaded Human Laminin 121 Protein, premium grade (Cat. No. LA1-H5261) on Protein A Biosensor, can bind Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) with an affinity constant of 15.7 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human Laminin 121 Protein, premium grade (Cat. No. LA1-H5261) on Protein A Biosensor, can bind Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) with an affinity constant of 6.95 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
