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| Items | Details |
| Detection Method | Chemiluminescence |
| Product Type | Magnetic Beads (Anti-Mouse IgG) |
| Quantity Size | 10mg / 100mg |
| Physical Appearance | lyophilized powder mixture |
| Particle size | 2.8 µm |
| Beads Surface | Hydrophilic |
| Amount of Coupled Protein | About 133 pmol (20 μg) Monoclonal Rabbit anti-mouse IgG antibody / mg Beads |
| Binding Capacity | 2-5ug mouse antibody or Mouse IgG Fc tag proteins / mg beads |
| Emission Wavelength | Measured relative light units (RLU) at 430 nm |
背景（Background）
The Anti-Mouse IgG-coupled Magnetic Beads are 2.8 µm superparamagnetic particles covalently coupled to a highly affinity Monoclonal Rabbit anti-mouse IgG antibody. The beads can be used to capture the mouse IgG in Chemiluminescence procedures. The antibody reacts with the heavy and light chain of mouse IgG. It can recognizes mouse IgG1, IgG2a, IgG2b and it binds IgG3 with a lower reactivity. It recognizes rat IgG and has no cross-reactivity with chicken IgY, goat IgG, human IgG, and rabbit IgG. The Anti-Mouse IgG-coupled Magnetic Beads is easy to capture the mouse IgG, and the bounded protein have no activity lost, this ready to use products could greatly save your protein coupling time and hassle, and help us get the best performance and highly reproducible results.
应用说明（Application）
The Anti-Mouse IgG-coupled Magnetic Beads is used to capture the mouse IgG, it can combination with Acridine ester markers in chemiluminescence technology, The Acridine ester markers such as Streptavidin-Acridine Ester can capture the biotinylated proteins or molecules, this allows detection of antigen and antibody binding or antibody screening.
重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
Upon receipt, please store the Beads at -20°C. The shelf life is 1 year at -20 °C.
Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
活性（Bioactivity）-MPCLIA
Immobilized 0.04 μg /Test of Biotinylated Human PD-1, Avitag,His Tag (Cat. No. PD1-H82E4) to the Streptavidin-Acridine ester (Cat. No. STN-NA114, 0.008 μg /Test), incubated with 100 μL /Test of Human PD-L1 Protein, Mouse IgG1 Fc Tag (Cat. No. PD1-H52A3) at increasing concentration coupled to Anti-Mouse IgG-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A003) (10 μg beads/Test). Detection was performed with sensitivity of 7.8 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
Protocol
The MPCLIA assay shows that Anti-Mouse IgG-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A003) is stable at 37°C for 10 days.
The MPCLIA assay shows that Anti-Mouse IgG-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A003) is stable after freezing and thawing 3 times.
The MPCLIA assay shows that Anti-Mouse IgG-coupled Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A003) is stable at 37°C for 10 days.
