膜杰作
Star Staining
| ID | Components | Size |
| RAB002-C01 | Pre-coated Human PD-1 Microplate | 1 plate |
| RAB002-C02 | Positive Control | 100 μL |
| RAB002-C03 | Negative Control | 100 μL |
| RAB002-C04 | HRP-Anti-Human IgG | 100 μL |
| RAB002-C05 | 10 x Washing Buffer | 50 mL |
| RAB002-C06 | Dilution Buffer | 50 mL |
| RAB002-C07 | Substrate Solution | 12 mL |
| RAB002-C08 | Stop Solution | 7 mL |
背景（Background）
Programmed cell death protein 1 (PD-1) is an inhibitory receptor that is expressed on some tumor cells and causes down regulation of the immune system by reducing T-cell activity. Anti-PD-1 monoclonal antibodies block the PD-1 receptor so the T cells are no longer inhibited and therefore activates the immune response against the tumor.
应用说明（Application）
The kit is developed for titer measurement of Anti-PD-1 Antibody IgG in Human serum.
It is for research use only.
存储（Storage）
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-PD-1 antibodies in Human serum. The Kit consists of Pre-coated with Human PD-1 Microplate , an Positive Control, an Negative Control, an HRP-Anti Human IgG secondary antibody.
Your experiment will include 4 simple steps:
a) The samples and Control are diluted by Dilution Buffer. Add your sample to the plate.
b) Add diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
