膜杰作
Star Staining
抗体来源（Source）
Monoclonal Anti-Human-IgG Antibody, Mouse IgG1 (6F11C8) antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Human-IgG.
亚型（Isotype）
Mouse IgG1 | Mouse Kappa
特异性（Specificity）
This product is a specific antibody specifically reacts with Human-IgG.
应用（Application）
ELISA
纯度（Purity）
>95% as determined by SDS-PAGE.
内毒素（Endotoxin）
Less than 1.0 EU per mg by the LAL method.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS,pH 7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Monoclonal Anti-Human-IgG-Fc Antibody,Mouse IgG1 (6F11C8) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-HPLC
The purity of Monoclonal Anti-Human-IgG-Fc Antibody,Mouse IgG1 (6F11C8) (Cat. No. IGG-AY69) was greater than >90% as determined by SEC-HPLC.
活性（Bioactivity）-ELISA
Immobilized Human ACE2 Protein, Fc Tag (Cat. No. AC2-H5257) at 2μg/mL (100μL/well) can bind Monoclonal Anti-Human-IgG-Fc Antibody,Mouse IgG1 (6F11C8) (HPLC verified) (Cat. No. IGG-AY69) with a linear range of 0.10-3.13 ng/mL (QC tested).
Protocol
Immobilized Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG1 (AM359b) (Cat. No. SPD-M265), Human IgG2 (AM359b) (Cat. No. SPD-M400a), Human IgG3 (AM359b) (Cat. No. SPD-M401a), Human IgG4 (AM359b) (Cat. No. SPD-M402a) and Human ACE2 / ACEH Protein, Fc Tag (Cat. No. AC2-H5257) can bind Monoclonal Anti-Human-IgG-Fc Antibody,Mouse IgG1 (6F11C8) (HPLC verified) (Cat. No. IGG-AY69). The antibody does not bind Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Cynomolgus IgG1 (AM122) (Cat. No. SPD-M201), Anti-SARS-CoV-2 Omicron Antibody-3A7C12, Rabbit IgG ( Cat. No. SPD-C73), Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgM (AM122) (Cat. No. SPD-M162), Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgA1 (AM130) (Cat. No. S1N-M164) and Human CD19 (20-291) Protein, His Tag DMF Filed (Cat. No. CD9-H52H2) (Routinely tested).
Protocol
背景（Background）
Immunoglobulins can be divided into five main classes/isotypes which are IgA, IgD, IgE, IgG, and IgM. IgG class identity is determined by class-specific sequences in the Fc region of the heavy chain. IgG antibody class are the most abundant immunoglobulins isotype in blood, lymph fluid, cerebrospinal fluid and peritoneal fluid. IgGs include four subclasses (IgG1, IgG2, IgG3, and IgG4). The IgG subclasses differ in their physical and chemical properties. Their distribution pattern is found to be age dependent and every subclass has a specific biological function.
