膜杰作
Star Staining
分子别名（Synonym）
Vinculin, VCL,MV,Metavinculin
表达区间及表达系统（Source）
Human Vinculin, His Tag (VIN-H52H9) is expressed from human 293 cells (HEK293). It contains AA Pro 2 - Gln 1066 (Accession # P18206-2).
Predicted N-terminus: Pro 2
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 118.5 kDa. The protein migrates as 130-145 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human Vinculin, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized Human Vinculin, His Tag (Cat. No. VIN-H52H9) at 1 μg/mL (100 μL/well) can bind Vinculin Antibody with a linear range of 0.2-16 ng/mL (QC tested).
Protocol
背景（Background）
Vinculin is a ubiquitously expressed actin-binding protein and involves in cross-linking of the cell cytoskeleton with the extracellular matrix for mechanical support of the cell. Vinculin is frequently used as a marker for both cell-cell and cell-extracellular matrix (focal adhesion) adherens-type junctions.
