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 >  Protein>IL-1 RAcP >ILP-H82F5

Biotinylated Human IL-1 RAcP / IL-1 R3 Protein, Fc,Avitag™ (MALS verified)

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分子别名(Synonym)

IL1RAP,IL-1RAcP,C3orf13,IL1R3

表达区间及表达系统(Source)

Biotinylated Human IL-1 RAcP Protein, Fc,Avitag (ILP-H82F5) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Glu 359 (Accession # Q9NPH3-1).

Predicted N-terminus: Ser 21

Request for sequence

蛋白结构(Molecular Characterization)

IL-1 RAcP Structure

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™)

The protein has a calculated MW of 66 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

IL-1 RAcP SDS-PAGE

Biotinylated Human IL-1 RAcP Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

IL-1 RAcP SEC-MALS

The purity of Biotinylated Human IL-1 RAcP Protein, Fc,Avitag (Cat. No. ILP-H82F5) is more than 85% and the molecular weight of this protein is around 155-175kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

IL-1 RAcP ELISA

Immobilized Human Anti-IL1RAP Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human IL-1 RAcP Protein, Fc,Avitag (Cat. No. ILP-H82F5) with a linear range of 0.5-16 ng/mL (QC tested).

Protocol

IL-1 RAcP ELISA

Immobilized Biotinylated Human IL-1 RAcP Protein, Fc,Avitag (Cat. No. ILP-H82F5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Anti-IL1RAP Human IgG1 with a linear range of 0.2-4 ng/mL (Routinely tested).

Protocol

 

背景(Background)

Interleukin-1 receptor accessory protein (IL1RAP or IL-1RAcP) is also known as Interleukin-1 receptor 3 (IL-1R-3 or IL-1R3), C3orf13, which belongs to the interleukin-1 receptor family. IL1RAP contains three Ig-like C2-type (immunoglobulin-like) domains and one TIR domain. IL1RAP is detected in liver, skin, placenta, thymus and lung. IL-1R3 / IL1RAP is coreceptor with IL1R1, associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways.

 

前沿进展

 
 
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IL-1 RAcP靶点信息
英文全称:Interleukin-1 receptor accessory protein
中文全称:
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:3详情
最高研发阶段:临床二期
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