Varicella Zoster Virus Glycoprotein E (VZV gE) ELISA Kit

背景（Background）

Varicella-zoster virus (VZV) the etiologic agent of chickenpox and herpes zoster [HZ], is highly contagious and still endemic worldwide. Glycoprotein E (gE) is one of the known glycoproteins (gB, gC, gE, gH, gI, gK, gI) of VZV that is most abundantly expressed on the surface of virus and infected cells, playing an important role in viral replication and cell-to-cell spread. The strongly immunogenic gE can provide strong IgG signal in body fluid, which makes it ideal to be developed as an antigen for analysis of Immunogenicity in the development of VZV vaccine.Therefore,It's helpful to develop the Varicella Zoster Virus Glycoprotein E (VZV gE) ELISA Kit to quantitative detection the VZV gE antigen in vaccine samples during the manufacture and quality control of vaccine development.

应用说明（Application）

This kit is developed for quantitative detection of Varicella Zoster Virus Glycoprotein E (VZV gE) in vaccine samples.

It is for research use only.

重构方法（Reconstitution）

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储 & 运输（Storage & Shipping）

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Shipping Statement

原理（Assay Principles）

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Glycoprotein E (VZV). The kit consists of Pre-coated Anti-Glycoprotein E (VZV) Antibody Microplate and Glycoprotein E (VZV) Standard and Biotin-Anti-Glycoprotein E (VZV) Antibody and Streptavidin-HRP and buffers.

Your experiment will include 6 simple steps:

a) All reagents were returned to room temperature(20°C-25°C) before use.

b) Add your sample to the plate, take the Glycoprotein E (VZV) as standard sample. The samples and standard sample are diluted by Dilution Buffer.

c) Wash the plate and add a diluted Biotin-Anti-Glycoprotein E (VZV) Antibody to the plate. The diluted by Dilution Buffer.

d) Wash the plate and add a diluted Streptavidin-HRP to the plate. The diluted by Dilution Buffer.

e) Wash the plate and add TMB.

f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.