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Dilution Buffer (Strengthen Blocking)

背景(Background)

Serum and some samples often cause nonspecific binding,especially after blocking,which lead to high background and low sensitivity.

应用说明(Application)

The buffer can reduce non-specific adsorption in experiments, avoid background signals which is caused by serum especially, achieve high signal-to-noise ratio, and achieve ideal detection sensitivity.

存储(Storage)

This product is stable after storage at: 2-8°C for 12 months: Opened kit is stable for up to 1 month from the date of opening.

 

活性(Bioactivity)-ELISA

- ELISA

2% serum diluted by Dilution Buffer : Biotinylated SARS-CoV-2 S protein RBD was put into, the background value is 2.383 and 2.148; 2% serum diluted by DB-02 : Biotinylated SARS-CoV-2 S protein RBD was put into, the background value is 0.262 and 0.263. In the existence of DB-02, the serum background values significantly decreased. Note: Dilution Buffer is Wash Buffer with 0.5% (w/v) bovine serum albumin (BSA), 50 mL.

Protocol

 
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