膜杰作
Star Staining
| Items | Size （2mg） | Size（5mg X 2） |
| Particle size | 2 μm | 2 μm |
| Physical appearance | Powder mixture | Powder mixture |
| Amount of Coupled Protein | ≈505pmol (70μg) Siglec-2 protein/mg beads | ≈505pmol (70μg) Siglec-2 protein/mg beads |
| Binding Capacity | >27pmol (2μg) Anti-hCD22 Ab/mg beads | >27pmol (2μg) Anti-hCD22 Ab/mg beads |
| Formulation | PBS, pH7.4, with 10% Trehalose | PBS, pH7.4, with 10% Trehalose |
| Reconstitution | 2mL sterile deionized water (1 mg beads/mL) | 5mL sterile deionized water (1 mg beads/mL) |
背景（Background）
The biotinylated CD22 protein was conjugated to streptavidin magnetic beads. This pre-coupled magnetic bead product can capture the anti-CD22 antibody from various assay systems. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This CD22 coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready-to-use product could greatly save your time and hassle.
表达区间及表达系统（Source）
Human CD22 Protein is expressed from human 293 cells (HEK293). It contains AA Asp 20 - Arg 687(Accession # P20273-1).
应用说明（Application）
This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
重构方法（Reconstitution）
See Certificate of Analysis (CoA) for detailed instruction.
存储（Storage）
Upon receipt, please store the Beads at -20°C. The shelf life is 1 year at -20 °C.
Please avoid more than 3 freeze-thaw cycles once reconstitution, immediate use after reconstitution is highly recommended.
原理（Assay Principles）
Antibody Purification: 1. Resuspend the lyophilized beads by adding the buffer of choice. 2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein. 3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads. 4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.
The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.
制剂（Formulation）
Please contact us for detailed information.
Contact us for customized product form or formulation.
典型数据-Typical Data
Immobilized 70μg Biotinylated Human CD22 protein to 1mg Beads can bind the Anti-CD22 Antibody with an EC50 0.7889 μg/mL.
Protocol
