Spike,S protein,Spike glycoprotein,S glycoprotein
Bat SARS-like coronavirus RsSHC014 Spike Trimer, His Tag (SPN-B52H8) is expressed from human 293 cells (HEK293). It contains AA Tyr 15 - Pro 1196 (Accession # U5WLK5(R668A, KA969-970PP)).
Predicted N-terminus: Tyr 15
Request for sequence
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 136.2 kDa. The protein migrates as 170-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Bat SARS-like coronavirus RsSHC014 Spike Trimer, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Bat SARS-like coronavirus RsSHC014 Spike Trimer, His Tag (Cat. No. SPN-B52H8) is more than 85% and the molecular weight of this protein is around 483-534 kDa verified by SEC-MALS.
Immobilized Bat SARS-like coronavirus RsSHC014 Spike Trimer, His Tag (Cat. No. SPN-B52H8) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-4 ng/mL (QC tested).
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.