膜杰作
Star Staining
分子别名（Synonym）
CEACAM-5,CD66e,CEA,Meconium antigen 100
表达区间及表达系统（Source）
Human CEACAM-5, Llama IgG2b Fc Tag (CE5-H5259) is expressed from human 293 cells (HEK293). It contains AA Pro 593 - Ser 675 (Accession # P06731-1).
Predicted N-terminus: Pro 593
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a llama IgG2b Fc tag at the C-terminus
The protein has a calculated MW of 36.7 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human CEACAM-5, Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human CEACAM-5, Llama IgG2b Fc Tag (Cat. No. CE5-H5259) is more than 85% and the molecular weight of this protein is around 80-108 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human CEACAM-5, Llama IgG2b Fc Tag (Cat. No. CE5-H5259) at 1 μg/mL (100 μL/well) can bind Anti-CEACAM-5 (labetuzumab) Antibody, Human IgG1 with a linear range of 0.01-0.313 μg/mL (QC tested).
Protocol
背景（Background）
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) is also Carcinoembryonic antigen (CEA), Meconium antigen 100, CD antigen CD66e, CEACAM5 belongs to the immunoglobulin superfamily and CEA family. CEACAM5 contains seven Ig-like (immunoglobulin-like) domains. CEACAM5 is homodimer protein which binding of E. coli Dr adhesins leads to dissociation of the homodimer. CEACAM5 is cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. CEACAM5 is receptor for E. coli Dr adhesins.
