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Human CEACAM-5 Protein, Llama IgG2b Fc Tag, low endotoxin (MALS verified)

分子别名(Synonym)

CEACAM-5,CD66e,CEA,Meconium antigen 100

表达区间及表达系统(Source)

Human CEACAM-5, Llama IgG2b Fc Tag (CE5-H5259) is expressed from human 293 cells (HEK293). It contains AA Pro 593 - Ser 675 (Accession # P06731-1).

Predicted N-terminus: Pro 593

Request for sequence

蛋白结构(Molecular Characterization)

Online(Pro 593 - Ser 675) P06731-1

This protein carries a llama IgG2b Fc tag at the C-terminus.

The protein has a calculated MW of 36.7 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.01 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human CEACAM-5, Llama IgG2b Fc Tag (Cat. No. CE5-H5259) SDS-PAGE gel

Human CEACAM-5, Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Human CEACAM-5, Llama IgG2b Fc Tag (Cat. No. CE5-H5259) MALS images

The purity of Human CEACAM-5, Llama IgG2b Fc Tag (Cat. No. CE5-H5259) is more than 85% and the molecular weight of this protein is around 80-108 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human CEACAM-5, Llama IgG2b Fc Tag (Cat. No. CE5-H5259) at 1 μg/mL (100 μL/well) can bind Anti-CEACAM-5 (labetuzumab) Antibody, Human IgG1 with a linear range of 0.01-0.313 μg/mL (QC tested).

Protocol

Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.

背景(Background)

Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) is also Carcinoembryonic antigen (CEA), Meconium antigen 100, CD antigen CD66e, CEACAM5 belongs to the immunoglobulin superfamily and CEA family. CEACAM5 contains seven Ig-like (immunoglobulin-like) domains. CEACAM5 is homodimer protein which binding of E.coli Dr adhesins leads to dissociation of the homodimer. CEACAM5 is cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. CEACAM5 is receptor for E.coli Dr adhesins.

 

前沿进展

 
 
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CEACAM-5靶点信息
英文全称:Carcinoembryonic antigen related cell adhesion molecule 5
中文全称:癌胚抗原相关细胞粘附分子5
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:12详情
最高研发阶段:临床三期
前沿进展

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