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Human Claudin-9 / CLDN9 Protein, His,Twin-Strep Tag (active membrane protein, SPR verified)

分子别名(Synonym)

CLDN9

表达区间及表达系统(Source)

Human Claudin-9, His,Twin-Strep Tag (CL9-H5586) is expressed from Baculovirus-Insect cells. It contains AA Ala 2 - Val 217 (Accession # O95484-1).

Predicted N-terminus: Met

Request for sequence

蛋白结构(Molecular Characterization)

Online(Ala 2 - Val 217) O95484-1

This protein carries a polyhistidine tag and a twin strep tag.

The protein has a calculated MW of 27.9 kDa. The protein migrates as 23-24 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.

This product is not suitable for cell based experiments due to cytotoxicity of DDM.

DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.

DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.

If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 12 months under sterile conditions.
*The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow this link for product information.
 

电泳(SDS-PAGE)

Human Claudin-9, His,Twin-Strep Tag (Cat. No. CL9-H5586) SDS-PAGE gel

Human Claudin-9, His,Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human Claudin-9, His,Twin-Strep Tag (Cat. No. CL9-H5586) at 10 μg/mL (100 μL/well) on an Nickel Coated plate can bind Anti-Claudin 9 Antibody, Rat IgG with a linear range of 0.01-0.156 μg/mL (QC tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Anti-Claudin 9 antibody captured on Protein G-Series S sensor chip can bind Human Claudin-9, His,Twin-Strep Tag (Cat. No. CL9-H5586) with an affinity constant of 38.8 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (QC tested).

Protocol

 

背景(Background)

Claudin-9 belongs to the claudin family. Claudins constitute integral membrane proteins responsible for solute and electrolyte permeability of the tight junction that serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Tight junctions also play a critical role in maintaining cell polarity and signal transductions. Claudin-9 creates charge specific channels in the paracellular space, plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity, is required to preserve sensory cells in the hearing organ because claudin-9-defective tight junctions fail to shield the basolateral side of hair cells from the K+-rich endolymph. Its ion barrier function is essential in the cochlea, but appears to be dispensable in other organs. Is one of the entry cofactors for hepatitis C virus; it enables HCV entry into target cells just as efficiently as CLDN1.

 

前沿进展

 
 

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