ID | Components | Size |
RAS122-C01 | Pre-coated Anti-Bevacizumab Antibody Microplate | 1 plate |
RAS122-C02 | Bevacizumab Standard | 100 μL |
RAS122-C03 | Biotin-Anti-Bevacizumab Antibody | 100 μL |
RAS122-C04 | Streptavidin-HRP | 50 μL |
RAS122-C05 | 10xWashing Buffer | 50 mL |
RAS122-C06 | 2xDilution Buffer | 50 mL |
RAS122-C07 | Substrate Solution | 12 mL |
RAS122-C08 | Stop Solution | 7 mL |
背景(Background)
A recombinant humanized monoclonal IgG1 antibody that binds to and inhibits the biologic activity of human vascular endothelial growth factor (VEGF). Bevacizumab contains human framework regions and the complementarity-determining regions of a murine antibody that binds to VEGF. Bevacizumab is produced in a Chinese Hamster Ovary mammalian cell expression system in a nutrient medium containing the antibiotic gentamicin and has a molecular weight of approximately 149 kilodaltons.
应用说明(Application)
The kit is developed for quantitative detection of Bevacizumab antibody in human serum.
It is for research use only.
存储 & 运输(Storage & Shipping)
Unopened kit should be stored at 2°C-8°C upon receiving.
Find the expiration date on the outside packaging and do not use reagents past their expiration date.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
The kit shipped at room temperature that had been validated. Please contact us if you need blue ice shipping, but additional freight may be followed.
Below is the Shipping Statement for Kit Products.
原理(Assay Principles)
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Bevacizumab. The kit consists of Pre-coated Anti-Bevacizumab Antibody Microplate and Bevacizumab Standard and Biotin-Anti-Bevacizumab Antibody and Streptavidin-HRP and buffers.
Your experiment will include 6 simple steps:
a) All reagents were returned to room temperature(20°C-25°C) before use.
b) Add your sample to the plate, take the Bevacizumab as standard sample. The samples and standard sample are diluted by Dilution Buffer.
c) Wash the plate and add a diluted Biotin-Anti-Bevacizumab Antibody to the plate. The diluted by Dilution Buffer.
d) Wash the plate and add a diluted Streptavidin-HRP to the plate. The diluted by Dilution Buffer.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.