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Anti-SARS-CoV-2 Omicron Antibody-3A7C12, Rabbit IgG

表达区间及表达系统(Source)

Anti-SARS-CoV-2 Omicron Antibody-3A7C12 is a chimeric monoclonal antibody recombinantly expressed from HEK293 cells, which combines the variable region of a mouse monoclonal antibody with rabbit Fc constant domain. The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Spike RBD, SARS-CoV-2.

亚型(Isotype)

Rabbit IgG | Kappa

特异性(Specificity)

This product is a specific antibody specifically reacts with SARS-CoV-2 Spike RBD, His Tag (B.1.1.529/Omicron). No cross-reactivity is detected with WT (Cat. No. SPD-C52H1), Alpha (Cat. No. SPD-C52Hn), Beta (Cat. No. SPD-C52Hp), Gamma (Cat. No. SPD-C52Hr) and Delta (Cat. No. SPD-C52Hh).

纯度(Purity)

>95% as determined by SDS-PAGE.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Anti-SARS-CoV-2 Omicron Antibody-3A7C12, Rabbit IgG (Cat. No. SPD-C73) SDS-PAGE gel

Anti-SARS-CoV-2 Omicron Antibody-3A7C12, Rabbit IgG on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized SARS-CoV-2 Spike RBD, His Tag (B.1.1.529/Omicron) (Cat. No. SPD-C522e) can bind Anti-SARS-CoV-2 Omicron Antibody-3A7C12, Rabbit IgG (Cat. No. SPD-C73) with a linear range of 0.39-6.25 ng/mL. The antibody does not bind WT (Cat. No. SPD-C52H1), Alpha (Cat. No. SPD-C52Hn), Beta (Cat. No. SPD-C52Hp), Gamma (Cat. No. SPD-C52Hr) and Delta (Cat. No. SPD-C52Hh) (QC tested).

Protocol

背景(Background)

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

 

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