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BCMA [Biotinylated] : BAFF Inhibitor Screening Chemiluminescence Assay Kit

For research use only.

IDComponentsSize
EP118-C01High-bind Plate1 plate
EP118-C02Human BAFF40 μg
EP118-C03Biotinylated Human BCMA10 μg
EP118-C04Anti-BCMA Neutralizing Antibody40 μg
EP118-C05Streptavidin-HRP10 μg
EP118-C06Coating Buffer12 mL
EP118-C0710xWashing Buffer 50 mL
EP118-C08Blocking Buffer50 mL
EP118-C09-AELISA Femto Substrate A6 mL
EP118-C09-BELISA Femto Substrate B6 mL

背景(Background)

TNFRSF17 is a member of the TNF-receptor family, known as tumor necrosis factor receptor superfamily member 17, also known as B cell maturation antigen (BCMA or BCM) or CD269. It is a protein encoded by TNFRSF17 gene.  The receptor is expressed on the surface of B lymphocytes and plasma cells and is a marker protein of B lymphocyte maturation.The main ligands of BCMA are BAFF and APRIL. BCMA combined with BAFF could activate the classical and non-classical NF-κB and JNK signaling pathways, up-regulate the expression of anti-apoptotic protein and down-regulate the expression of pro-apoptotic protein. When BCMA binds to APRIL, the expression level of immune checkpoint is up-regulated, creating an immunosuppressed bone marrow microenvironment.

应用说明(Application)

This kit is developed for screening for inhibitors of human BCMA binding to human BAFF.

It is for research use only.

重构方法(Reconstitution)

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储(Storage)

The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

原理(Assay Principles)

This inhibitor screening Assay kit is designed to facilitate the identification and characterization of new BCMA pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human BCMA to immobilized human BAFF in a functional chemiluminescence assay. Briefly, we provide you with a human Biotinylated BCMA protein, a human BAFF protein, an anti-BCMA neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.

Your experiment will include 4 simple steps:

a) Coat the plate with human BAFF.

b) Add your molecule of interest to the tests.

c) Add human BCMA-Biotin to bind the coated human BAFF.

d) Add Streptavidin-HRP followed by luminol .

Finally, the half maximal inhibitory concentration (IC50) of your compound to BCMA: BAFF binding will be determined by comparing RLU readings among different experimental groups.

 

典型数据-Typical Data

BCMA ELISA

INHIBITION OF BCMA [BIOTINYLATED]: BAFF BINDING BY ANTI-BCMA NEUTRALIZING ANTIBODY
Serial dilutions of Anti-BCMA Neutralizing antibody (Catalog # EP118-C04) (1:1 serial dilution, from 20 μg/mL to 0.02μg/mL) was added into Biotinylated BCMA: BAFF binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

Protocol

前沿进展

 
 
货号/价格
文档
联系电话:
400-682-2521(全国)
010-53681107(北京)
021-31168505(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
BCMA靶点信息
英文全称:B-cell maturation protein
中文全称:B细胞成熟蛋白
种类:Homo sapiens
上市药物数量:2详情
临床药物数量:58详情
最高研发阶段:批准上市
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