膜杰作
Star Staining
分子别名（Synonym）
Coagulation factor Ix,PTC,F9,THPH8,FIX,Plasma Thromboplastic Component,factor Ix
表达区间及表达系统（Source）
Cynomolgus Coagulation factor IX, His Tag (FIX-C52H9) is expressed from human 293 cells (HEK293). It contains AA Thr 29 - Thr 461 (Accession # G7Q1U2-1).
Predicted N-terminus: Thr 29
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 50.6 kDa. The protein migrates as 61-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl,pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Cynomolgus Coagulation factor IX, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
检测平台（SEC-MALS）
The purity of Cynomolgus Coagulation factor IX, His Tag(Cat. No. FIX-C52H9) is more than 90% and the molecular weight of this protein is around 55-75 kDa verified by SEC-MALS.
Report
活性（Bioactivity）
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 . The specific activity is >30 pmol/min/μg (QC tested).
