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 >  低内毒素蛋白>NKG2D >NKD-H5257

Human NKG2D / CD314 Protein, Llama IgG2b Fc Tag, low endotoxin

分子别名(Synonym)

NKG2D,CD314,KLRK1,NK cell receptor D

表达区间及表达系统(Source)

Human NKG2D, Llama IgG2b Fc Tag (NKD-H5257) is expressed from human 293 cells (HEK293). It contains AA Ile 73 - Val 216 (Accession # P26718-1).

Predicted N-terminus: Glu

Request for sequence

蛋白结构(Molecular Characterization)

Online(Ile 73 - Val 216) P26718-1

This protein carries a llama IgG2b Fc tag at the N-terminus.

The protein has a calculated MW of 44.5 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.01 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human NKG2D, Llama IgG2b Fc Tag (Cat. No. NKD-H5257) SDS-PAGE gel

Human NKG2D, Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human MICA, His Tag (Cat. No. MIA-H5221) at 5 μg/mL (100 μL/well) can bind Human NKG2D, Llama IgG2b Fc Tag (Cat. No. NKD-H5257) with a linear range of 0.01-0.625 μg/mL (QC tested).

Protocol

Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.

背景(Background)

NKG2D is a transmembrane protein belonging to the CD94/NKG2 family of C-type lectin-like receptors, also known as KLRK1, CD314, D12S2489E, KLR and killer cell lectin like receptor K1. NKG2D itself forms a homodimer whose ectodomains serve for ligand binding. NKG2D is a major recognition receptor for the detection and elimination of transformed and infected cells as its ligands are induced during cellular stress, either as a result of infection or genomic stress such as in cancer. In NK cells, NKG2D serves as an activating receptor, which itself is able to trigger cytotoxicity. The function of NKG2D on CD8+ T cells is to send co-stimulatory signals to activate them.

 

 
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NKG2D靶点信息
英文全称:NKG2-D type II integral membrane protein
中文全称:
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:4详情
最高研发阶段:临床二期
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