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Streptavidin Protein-Alexa Fluor™ 555 (HPLC verified)

分子别名(Synonym)

Streptavidin,SA

表达区间及表达系统(Source)

Streptavidin Protein-Alexa Fluor 555 (STN-NA115) is expressed from E. coli cells.

蛋白结构(Molecular Characterization)

This protein carries no "tag".

The protein has a calculated MW of 13.8 kDa. The protein migrates as 15 kDa under reducing (R) condition (SDS-PAGE).

偶联(Conjugate)

AF555

Excitation Wavelength: 561 nm

Emission Wavelength: 572 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with AF555 using standard chemical labeling method. The residual AF555 is removed by molecular sieve treatment during purification process.

蛋白标记度(Protein Ratio)

The AF555 to protein molar ratio is 2-3.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-HPLC.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Streptavidin SDS-PAGE

Streptavidin Protein-Alexa Fluor 555 on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC

Streptavidin SEC-HPLC

The purity of Streptavidin Protein-Alexa Fluor 555 (Cat. No. STN-NA115) was greater than 95% as determined by SEC-HPLC.

 

背景(Background)

Streptavidin is a 66KDa tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity to biotin. Each unit can bind one biotin. Horseradish peroxidase is metalloenzyme, a 44KDa glycoprotein. When incubate with substrates, it produces a coloured, fluorimetric, or luminescent derivatives, which can be detected and quantified. HRP conjugated Streptavidin is widely used for the detection and quantification of biotinylated proteins.

 

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