膜杰作
Star Staining
| ID | Components | Size |
| EP132-C01 | High-bind Plate | 1 plate |
| EP132-C02 | Human IL-4 R alpha | 30 μg |
| EP132-C03 | Human IL-4 | 20 μg |
| EP132-C04 | Human IL-4-Biotin | 10 μg |
| EP132-C05 | Streptavidin-HRP | 10 μg |
| EP132-C06 | Coating Buffer | 12 mL |
| EP132-C07 | 10xWashing Buffer | 50 mL |
| EP132-C08 | Blocking Buffer | 50 mL |
| EP132-C09 | Substrate Solution | 12 mL |
| EP132-C10 | Stop Solution | 7 mL |
Background/背景
Interleukin-4, is a cytokine that induces differentiation of naive helper T cells (Th0 cells to Th2 cells). In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine.
Application/应用
This kit is developed for screening for inhibitors of human IL-4Rα binding to human IL-4.
It is for research use only.
Reconstitution/重构方法
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage/存储
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Assay Principles/原理
This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new IL-4 pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated human IL-4 to immobilized human IL-4 R alpha in a functional ELISA assay, and employs a simple colorimetric sandwich ELISA platform.
Your experiment will include 4 simple steps:
a) Coat the plate with human IL-4 R alpha.
b) Add your molecule of interest to the tests.
c) Add human IL-4-Biotin to bind the coated human IL-4 R alpha.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the ability of your compound to inhibit IL-4: IL-4 R alpha Rbinding will be determined by comparing OD readings among different experimental groups.
Typical Data/典型数据
INHIBITION OF IL-4 [BIOTINYLATED]: IL-4 R ALPHA BINDING BY Human IL-4
Serial dilutions of Human IL-4(Catalog # EP132-C03) (1:1 serial dilution, from 5 μg/mL to 0.01 μg/mL (295.86-0.58 nM)) was added into IL-4 R alpha: IL-4-Biotin binding reactions. The assay was performed according to the above-described protocol. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
Protocol
