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Biotinylated Human NPR1 / NPRA Protein, His,Avitag™ (MALS verified)

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分子别名(Synonym)

Atrial natriuretic peptide receptor 1,NPR1,NPRA,GC-A,ANPRA,NPR-A

表达区间及表达系统(Source)

Biotinylated Human NPR1, His,Avitag (NP1-H82E9) is expressed from human 293 cells (HEK293). It contains AA Gly 33 - Glu 473 (Accession # P16066-1).

Predicted N-terminus: Gly 33

Request for sequence

蛋白结构(Molecular Characterization)

Online(Gly 33 - Glu 473) P16066-1

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 52.5 kDa. The protein migrates as 63-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Biotinylated Human NPR1, His,Avitag (Cat. No. NP1-H82E9) SDS-PAGE gel

Biotinylated Human NPR1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Biotinylated Human NPR1, His,Avitag (Cat. No. NP1-H82E9) MALS images

The purity of Biotinylated Human NPR1, His,Avitag (Cat. No. NP1-H82E9) is more than 85% and the molecular weight of this protein is around 65-80 kDa verified by SEC-MALS.

Report

背景(Background)

Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.

 

 

前沿进展

 
 
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NPR1靶点信息
英文全称:Natriuretic peptide receptor 1
中文全称:利钠肽受体1
种类:
上市药物数量:3详情
临床药物数量:9详情
最高研发阶段:批准上市
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