膜杰作
Star Staining
分子别名（Synonym）
KLRG1
表达区间及表达系统（Source）
Mouse KLRG1, His Tag (KL1-M5249) is expressed from human 293 cells (HEK293). It contains AA Gln 57 - Tyr 188 (Accession # O88713-1).
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蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus
The protein has a calculated MW of 17.1 kDa. The protein migrates as 24-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Mouse KLRG1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Mouse KLRG1, His Tag (Cat. No. KL1-M5249) is more than 85% and the molecular weight of this protein is around 19-27 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-SPR
Mouse KLRG1, His Tag (Cat. No. KL1-M5249) immobilized on CM5 Chip can bind Human E-Cadherin, Fc Tag, premium grade (Cat. No. ECD-H5250) with an affinity constant of 0.699 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
背景（Background）
The co-inhibitory receptor killer-cell lectin like receptor G1 (KLRG1) is specifically expressed on NK cells and activated CD8+ T-cells and has been postulated to be a marker of senescence. KLRG1+ T cells are a major reason of chronic tissue damage in some autoimmune diseases such as systemic lupus erythematosus and rheumatoid arthritis. In tumors, tumor cells which express E-cadherin or N-cadherin bind to KLRG1 and inhibit the antitumor activity of T and NK cells. Thus, KLRG1 acts as an immunocheckpoint inhibitory receptor.
