分子别名(Synonym)
Integrin alpha 11 beta 1,ITGA11&ITGB1
表达区间及表达系统(Source)
Canine ITGA11&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-C52W9) is expressed from human 293 cells (HEK293). It contains AA Asp 21 - Pro 1142 (ITGA11) & Asp 23 - Asp 728 (ITGB1) (Accession # A0A8I3S131-1 (ITGA11) & A0A8C0PYR6-1 (ITGB1)).
Predicted N-terminus: Asp 21 (ITGA11) & Asp 23 (ITGB1)
Request for sequence
蛋白结构(Molecular Characterization)
Canine ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA1 and ITGB1, has a calculated MW of 132.6 kDa (ITGA11) and 83.2 kDa (ITGB1). Subunit ITGA1 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>90% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Canine ITGA11&ITGB1 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性(Bioactivity)-ELISA
Immobilized Native Human Collagen I protein at 10 μg/mL (100 μL/well) can bind Canine ITGA11&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-C52W9) with a linear range of 0.078-5 μg/mL (QC tested).
Protocol
Immobilized Native Human Collagen IV Protein at 10 μg/mL (100 μL/well) can bind Canine ITGA11&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-C52W9) with a linear range of 0.01-0.313 μg/mL (Routinely tested).
Protocol
背景(Background)
Integrin alpha 11 beta 1 is a receptor for collagen. The alpha11 cDNA encodes a mature protein with a large 1120-residue extracellular domain that contains an I-domain of 207 residues and is linked by a transmembrane domain to a short cytoplasmic domain of 24 amino acids. The deduced alpha11 protein shows the typical structural features of integrin alpha-subunits and is similar to a distinct group of alpha-subunits from collagen-binding integrins.