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Biotinylated Human CCL1 / I-309 / C-C motif chemokine 1 Protein, His,Avitag™ (MALS verified)

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分子别名(Synonym)

CCL1,I-309,C-C motif chemokine 1

表达区间及表达系统(Source)

Biotinylated Human CCL1, His,Avitag (CC1-H82E9) is expressed from human 293 cells (HEK293). It contains AA Lys 24 - Lys 96 (Accession # P22362-1 ).

Predicted N-terminus: Lys 24

Request for sequence

蛋白结构(Molecular Characterization)

CCL1 Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 12.1 kDa. The protein migrates as 17-19 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

CCL1 SDS-PAGE

Biotinylated Human CCL1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

CCL1 SEC-MALS

The purity of Biotinylated Human CCL1, His,Avitag (Cat. No. CC1-H82E9) is more than 90% and the molecular weight of this protein is around 18-26 kDa verified by SEC-MALS.

Report

 

背景(Background)

Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL1, a member of the CC subfamily, is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine (C-C motif) receptor 8.

 

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