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Biotinylated Human HLA-A*11:01&B2M Monomer Protein (Peptide free, MALS verified)

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分子别名(Synonym)

HLA-A*11:01 & B2M

表达区间及表达系统(Source)

Biotinylated Human HLA-A*11:01&B2M Monomer Protein (HLM-H82W5) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) (Accession # AAV53343.1 (HLA-A*11:01) & P61769 (B2M)).

Predicted N-terminus: Gly 25 & Ile 21

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 36.0 kDa and 11.7 kDa. The protein migrates as 42-45 kDa and 13 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

HLA-A*11:01 & B2M SDS-PAGE

Biotinylated Human HLA-A*11:01&B2M Monomer Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

HLA-A*11:01 & B2M SEC-MALS

The purity of Biotinylated Human HLA-A*11:01&B2M Monomer Protein (Cat. No. HLM-H82W5) is more than 90% and the molecular weight of this protein is around 40-60 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

HLA-A*11:01 & B2M ELISA

Immobilized Biotinylated Human HLA-A*11:01&B2M Monomer Protein (Cat. No. HLM-H82W5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.00003-0.008 ng/mL (QC tested).

Protocol

HLA-A*11:01 & B2M ELISA

Immobilized Biotinylated Human HLA-A*11:01&B2M Monomer Protein (Cat. No. HLM-H82W5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).

Protocol

 

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