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 >  Protein>Tau >TAU-H5115

Human Tau-441 / 2N4R Pre-formed Fibrils Protein, Tag Free

分子别名(Synonym)

DDPAC,FTDP-17,MAPT,MSTD,MTBT1,Tau,PHF-tau,TAU

表达区间及表达系统(Source)

Human Tau-441 Pre-formed Fibrils, Tag Free (TAU-H5115) is expressed from E. coli cells. It contains AA Met 1 - Leu 441 (Accession # P10636-8).

Predicted N-terminus: Met 1

Request for sequence

蛋白结构(Molecular Characterization)

Tau Structure

This protein carries no "tag".

The protein has a calculated MW of 45.8 kDa.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Supplied as 0.2 μm filtered solution in PBS, pH7.4.

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储(Storage)

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product should be stored at -70°C or room temperature for short storage. Do not store fibrils on ice or at 4°C;
  2. The unsonicated fibril is validated to be stable after storage at -70°C for 1 year under sterile conditions;
  3. The sonicated fibril should be stored at -70°C for not more than 8 weeks.
 

电泳(SDS-PAGE)

Tau SDS-PAGE

Tau-441 monomer on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ThT Assay

Tau THT

Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time when tau wild-type monomers (Cat. No. TAU-H5117) are combined with tau wild-type Pre-formed Fibrils (Cat. No. TAU-H5115) (Routinely tested).

Protocol

 

电镜(Electron Microscope)

Tau ELECTRON MICROSCOPE

Transmission electron microscopy (TEM) of Tau-441 preformed fibrils (Cat. No. TAU-H5115). Fibril structure is visible on negative stain TEM images of TAU-H5115 (Routinely tested).

 

活性(Bioactivity)-Bioactivity CELL BASE

Tau CELL

HEK293/Human Tau (GFP) Stable Cell Line (Cat. No. CHEK-ATP087) were transduced with Human Tau-441 Pre-formed Fibrils, Tag Free (Cat. No. TAU-H5115) and Human Tau-441, Tag Free (Cat. No. TAU-H5117) respectively. The fluorescence of GFP-Tau (Green) and DAPI (Blue) were detected by confocal microscope. A. Lipo2000 transduction. B. Lipo2000 and Human Tau-441, Tag Free transduction. C. Lipo2000 and Human Tau-441 Pre-formed Fibrils, Tag Free transduction. Scale bars, 50 μm (Routinely tested).

Protocol

 

背景(Background)

Tau is a microtubule-associated protein, which encodes by the MAPT gene that located on chromosome 17q21. Tau Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. Hyperphosphorylation of the tau protein (tau inclusions, pTau) can result in the self-assembly of tangles of paired helical filaments and straight filaments, which are involved in the pathogenesis of Alzheimer's disease, frontotemporal dementia, and other tauopathies.

 

前沿进展

 
 
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Tau靶点信息
英文全称:Microtubule-associated protein tau
中文全称:微管相关蛋白tau
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:36详情
最高研发阶段:批准上市
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