膜杰作
Star Staining
分子别名（Synonym）
FCGR1A,FCG1,FCGR1,IGFR1,CD64,CD64A,FCRI
表达区间及表达系统（Source）
Canine CD64, His Tag (FCA-C52H8) is expressed from human 293 cells (HEK293). It contains AA Gln 16 - Pro 288 (Accession # XP_025308990.1).
Predicted N-terminus: Gln 16
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 32.4 kDa. The protein migrates as 36-46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Canine CD64, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Canine CD64, His Tag (Cat. No. FCA-C52H8) is more than 90% and the molecular weight of this protein is around 33-49 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-SPR
Canine CD64, His Tag (Cat. No. FCA-C52H8) captured on CM5 chip via anti-His antibody can bind Herceptin with an affinity constant of 2.38 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Canine CD64, His Tag (Cat. No. FCA-C52H8) captured on CM5 chip via anti-His antibody can bind Ipilimumab with an affinity constant of 2.30 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Canine CD64, His Tag (Cat. No. FCA-C52H8) captured on CM5 chip via anti-His antibody can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) with an affinity constant of 0.769 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
背景（Background）
Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses.
High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells.
