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Anti-SARS-CoV-2 Antibody IgG Quantitative and Titer Detection Kit (Spike RBD)

For research use only.

IDComponentsSize
RAS093-C01Pre-coated SARS-CoV-2 Spike RBD Microplate1 plate
RAS093-C02SARS-CoV-2 Antibody Positive Control100 μL
RAS093-C03SARS-CoV-2 Antibody Negative Control100 μL
RAS093-C04Calibrator10.5 mL
RAS093-C05Calibrator20.5 mL
RAS093-C06Calibrator30.5 mL
RAS093-C07Calibrator40.5 mL
RAS093-C08Calibrator50.5 mL
RAS093-C09Calibrator60.5 mL
RAS093-C10HRP-Anti-Human IgG10 μg
RAS093-C1110 x Washing Buffer 50 mL
RAS093-C12Dilution Buffer50 mL
RAS093-C13Substrate Solution12 mL
RAS093-C14Stop Solution7 mL

背景(Background)

The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective Assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.

应用说明(Application)

This kit is developed for quantitative or titer detection of Anti-SARS-CoV-2 Antibody IgG (Spike RBD) in human serum.

It is for research use only.

重构方法(Reconstitution)

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储 & 运输(Storage & Shipping)

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Shipping Statement

原理(Assay Principles)

This assay kit employs a standard indirect-ELISA format, providing a rapid detection of anti-SARS-CoV-2 IgG in serum by SARS-CoV-2 Spike RBD. The kit consists of Pre-coated SARS-CoV-2 Spike RBD Microplate, an Positive control,Negative Control, Calibrator,an HRP-Anti-Human IgG secondary antibody and Dilution buffer.

Your experiment will include 4 simple steps: a) Add your sample and Calibrator to the plate. The samples are diluted by Dilution Buffer.

b) Add a diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

c) Wash the plate and add TMB or other colorimetric HRP substrate.

d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

 

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