膜杰作
Star Staining
分子别名（Synonym）
CLEC-2,CLEC1B,C-type Lectin-like Receptor 2
表达区间及表达系统（Source）
Human CLEC-2, Fc Tag (CL2-H5254) is expressed from human 293 cells (HEK293). It contains AA Ser 55 - Pro 229 (Accession # Q9P126-1).
Predicted N-terminus: Ser 55
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus
The protein has a calculated MW of 47.2 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human CLEC-2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human CLEC-2, Fc Tag (Cat. No. CL2-H5254) is more than 90% and the molecular weight of this protein is around 100-125 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human Podoplanin, His Tag (Cat. No. PON-H52H3) at 5 μg/mL (100 μL/well) can bind Human CLEC-2, Fc Tag (Cat. No. CL2-H5254) with a linear range of 0.313-5 μg/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Human Podoplanin, His Tag (Cat. No. PON-H52H3) immobilized on CM5 Chip can bind Human CLEC-2, Fc Tag (Cat. No. CL2-H5254) with an affinity constant of 0.495 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
背景（Background）
C-type lectin-like receptor 2 (CLEC-2) is a hemITAM containing receptor that is highly expressed on platelets and regulates many of the non-classical functions of these cells, including in inflammation and infection.
