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Human Siglec-7 / CD328 Protein, Fc (L117A, L118A) Tag (MALS verified)

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分子别名(Synonym)

CDw328,D-siglec,A79 membrane protein,p75,Adhesion inhibitory receptor molecule 1, AIRM-1

表达区间及表达系统(Source)

Human Siglec-7, Fc (L117A, L118A) Tag (SI7-H5258) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Leu 353 (Accession # Q9Y286-1 ).

Predicted N-terminus: Gln 19

Request for sequence

蛋白结构(Molecular Characterization)

Siglec-7 Structure

This protein carries a Fc (L117A, L118A) tag at the C-terminus. The protein has a calculated MW of 63.3 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Mutations (L117A, L118A/EU number: L234A/L235A) in human immunoglobulin G1 (hIgG1) Fc diminish binding to FcγRI leading to a significant reduction in ADCC and CDC in comparison to wild type human IgG1.

内毒素(Endotoxin)

Less than 0.01 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, pH5.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Siglec-7 SDS-PAGE

Human Siglec-7, Fc (L117A, L118A) Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Siglec-7 SEC-MALS

The purity of Human Siglec-7, Fc (L117A, L118A) Tag (Cat. No. SI7-H5258) is more than 85% and the molecular weight of this protein is around 140-170 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Siglec-7 ELISA

Immobilized Human Siglec-7, Fc (L117A, L118A) Tag (Cat. No. SI7-H5258) at 5 μg/mL (100 μL/well) can bind Neu5Aca2-6GalNAca-PAA-biotin with a linear range of 0.01-0.625 μg/mL (QC tested).

Protocol

 

背景(Background)

Siglec-7 is a member of the human CD33‐related Siglec receptor. The extracellular region of Siglec‐7 is characterized by an N‐terminal V‐set Ig domain that can bind sialic acid and two C2‐set Ig domains. The cytoplasmic tail of Siglec‐7 has one immune‐receptor tyrosine‐based inhibitory motif (ITIM) and one ITIM‐like motif. Siglec-7 is considered as a sialic acid-dependent immunoreceptor with inhibitory potential and expressed predominantly on human NK cells, monocytes and a small subset of CD8+ T cells.

 

前沿进展

 
 
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Siglec-7靶点信息
英文全称:Sialic acid-binding Ig-like lectin 7
中文全称:唾液酸结合免疫球蛋白样凝集素-7
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:0详情
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