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Mouse APP / N-APP Protein, His Tag (MALS verified)

分子别名(Synonym)

ABPP,APPI,Amyloid-beta A4 protein

表达区间及表达系统(Source)

Mouse N-APP, His Tag (APP-M52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 18 - Val 286 (Accession # P12023-1).

Predicted N-terminus: Leu 18

Request for sequence

蛋白结构(Molecular Characterization)

Online(Leu 18 - Val 286) P12023-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 31.9 kDa. The protein migrates as 38-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Mouse N-APP, His Tag (Cat. No. APP-M52H3) SDS-PAGE gel

Mouse N-APP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Mouse N-APP, His Tag (Cat. No. APP-M52H3) MALS images

The purity of Mouse N-APP, His Tag (Cat. No. APP-M52H3) is more than 90% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.

Report

背景(Background)

Amyloid precursor protein (APP) is a type I integral membrane protein ubiquitously expressed in many tissues and concentrated in the synapses of neurons. The majority of APP is cleaved at the plasma membrane by the α-secretase in the non-amyloidogenic pathway. The amyloidogenic pathway starts with β-secretase cleavage by BACE1 on the N-terminal part of the Aβ domain, releasing sAPPβ from a membrane-anchored fragment named βCTF or C102, which is subsequently cleaved by γ-secretase to release Aβ.

 

 

前沿进展

 
 
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APP靶点信息
英文全称:Beta amyloid A4 protein
中文全称:β-淀粉状蛋白A4
种类:Homo sapiens
上市药物数量:11详情
临床药物数量:44详情
最高研发阶段:批准上市
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