膜杰作
Star Staining
分子别名（Synonym）
Glial fibrillary acidic protein
表达区间及表达系统（Source）
Human GFAP, His Tag (GFP-H5143) is expressed from E. coli cells. It contains AA Met 1 - Met 432 (Accession # P14136-1).
Predicted N-terminus: Met
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus
The protein has a calculated MW of 51.9 kDa. The protein migrates as 50 kDa under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in 20 mM Tris, 500 mM NaCl, 0.5 M Arginine, pH8.0 with trehalose as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
电泳（SDS-PAGE）
Human GFAP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
活性（Bioactivity）-ELISA
Immobilized Human GFAP, His Tag (Cat. No. GFP-H5143) at 1 μg/mL (100 μL/well) can bind GFAP Monoclonal Antibody with a linear range of 0.8-25 ng/mL (QC tested).
Protocol
背景（Background）
GFAP (Glial fibrillary acidic protein) is a monomeric intermediate filament protein fuond in mature astrocytes and other glial cells but is not found outside the CNS. Increased GFAP immunoreactivity (or astrocytic activation) is usually viewed as an index of gliosis or a relatively slow-developing correlate of neural damage.
