膜杰作
Star Staining
分子别名（Synonym）
CA125,CA-125,CA125MUC-16,FLJ14303,MUC16,mucin 16,mucin-16
表达区间及表达系统（Source）
Rhesus macaque CA125 (12664-12920), His Tag (CA5-R52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 12664 - Val 12920 (Accession # XP_015296314.1).
Predicted N-terminus: Gly 12664
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 29.9 kDa. The protein migrates as 44-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.1 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Rhesus macaque CA125 (12664-12920), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Rhesus macaque CA125 (12664-12920), His Tag (Cat. No. CA5-R52H3) is more than 90% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Rhesus macaque CA125 (12664-12920), His Tag (Cat. No. CA5-R52H3) at 5 μg/mL (100 μL/well) can bind Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H5253) with a linear range of 1-20 ng/mL (QC tested).
Protocol
背景（Background）
The CA125, also known as the MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. The CA 125 molecule is almost certainly a glycoprotein with a predominance of O-linkages. It is heterogeneous with regard to both size and charge, most likely due to continuous deglycosylation of side chains during its life-span in bodily fluids. It exists as a very large complex (perhaps as much as 4 million daltons) under natural conditions.
