登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>IL-3 R alpha >ILA-HP2H8

PE-Labeled Human IL-3 R alpha / CD123 Protein, His Tag (Site-specific conjugation)

分子别名(Synonym)

IL3R,IL3RA,IL-3Ra,IL-3R-alpha,IL3RAY,IL3RX,IL3RY,CD123 antigen,CD123,hIL3Ra,hIL-3Ra,MGC34174,IL-3 R alpha

表达区间及表达系统(Source)

PE-Labeled Human IL-3 R alpha, His Tag (ILA-HP2H8) is produced via site-specific conjugation of PE to Human IL-3 R alpha, His Tag under optimal conditions with a proprietary technology. Human IL-3 R alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Thr 19 - Arg 305  (Accession # P26951-1).

Predicted N-terminus: Thr 19 

Request for sequence

蛋白结构(Molecular Characterization)

Online(Thr 19 - Arg 305 ) P26951-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 36.7 kDa.

应用说明(Application)

Please note that this product is NOT compatible to streptavidin detection system.

Conjugate

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.

活性(Bioactivity)-FACS

Biotinylated Human FACS

5e5 of anti-IL-3 R alpha CAR-293 cells were stained with 100 μL of 1:25 dilution (4 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human IL-3 R alpha, His Tag (Cat. No. ILA-HP2H8) and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).

Protocol

 

背景(Background)

Interleukin 3 receptor alpha (low affinity) (IL3RA), also known as CD123 (Cluster of Differentiation 123) is a 70-kD glycoprotein member of the hematopoietin receptor superfamily. This protein associates with a beta subunit common to the receptors for IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to form a high-affinity receptor for IL-3. The interleukin-3 receptor α chain (CD123) has been identified as a potential immunotherapeutic target because it is overexpressed in AML compared with normal hematopoietic stem cells.

 

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
IL-3 R alpha靶点信息
英文全称:Interleukin-3 receptor subunit alpha
中文全称:白细胞介素-3受体α亚基
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:33详情
最高研发阶段:批准上市
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定