登录 | 注册    关注公众号  
微信公众号
搜索
 >  Antibody>Spike RBD >SPD-M402a

Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG4 (AM359b) (MALS verified)

抗体来源(Source)

The antibody is isolated from the serum of COVID-19 vaccine recipient and is recombinantly produced from human 293 cells (HEK293). This antibody can broadly neutralize SARS-CoV-2 Variants of Concerns (VOCs) including Alpha, Beta, Gamma, Delta and Omicron.

亚型(Isotype)

Human IgG4/kappa

特异性(Specificity)

This product is a specific antibody against SARS-CoV-2 Spike protein RBD domain. Cross-reactivity with Spike protein RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1 has not been tested.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Spike RBD SDS-PAGE

Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG4 (AM359b) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Spike RBD SEC-MALS

The purity of Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG4 (AM359b) (Cat. No. SPD-M402a) is more than 90% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Spike RBD ELISA

Immobilized SARS-CoV-2 Spike Trimer, His Tag (B.1.1.529/Omicron) (Cat. No. SPN-C52Hz) at 2μg/mL (100μL/well) can bind Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG4 (AM359b) (Cat. No. SPD-M402a) with a linear range of 0.2-6 ng/mL (QC tested).

Protocol

Spike RBD ELISA

Immobilized SARS-CoV-2 Spike RBD, His Tag (B.1.1.529/Omicron) (Cat. No. SPD-C522e) at 2μg/mL (100μL/well) can bind Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG4 (AM359b) (Cat. No. SPD-M402a) with a linear range of 0.1-3 ng/mL (Routinely tested).

Protocol

Spike RBD ELISA

Immobilized SARS-CoV-2 Spike protein WT (Cat. No. SPN-C52H9), Alpha (Cat. No. SPN-C52H6), Beta (Cat. No. SPN-C52Hk),Gamma (Cat. No. SPN-C52Hg), Delta (Cat. No. SPN-C52He) and Omicron (Cat. SPN-C52Hz) can bind Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG4 (AM359b) (Cat. No. SPD-M402a) with a linear range of 0.20-6.25 ng/mL (Routinely tested).

Protocol

Spike RBD ELISA

Immobilized SARS-CoV-2 Spike RBD WT (Cat. No. SPD-C52H1), Delta (Cat. No. SPD-C52Hh) and Omicron (Cat. SPD-C522e) can bind Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG4 (AM359b) (Cat. No. SPD-M402a) with a linear range of 0.20-6.25 ng/mL (Routinely tested).

Protocol

Spike RBD ELISA

Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG4 (AM359b) (Cat.No. SPD-M402a) neutralizes SARS-CoV-2 Spike Trimer by inhibiting Spike: ACE2 interaction. The Wild type (WT) Spike Trimer or Alpha, Delta, B.1.1.529/Omicron, BA.2/Omicron, BA.3/Omicron mutant-coated plate is incubated with the Biotinylated Human ACE2 / ACEH Protein and treated with the neutralizing antibody at increasing concentration . Percent inhibition is calculated based on the OD value.

Protocol

背景(Background)

Its been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

 

文献引用(Citations)

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定