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Human Amyloid-like protein 2 / APLP2 Protein, His Tag (MALS verified)

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分子别名(Synonym)

Amyloid beta precursor like protein 2,APLP2,APPH,CDEBP,APPL2

表达区间及表达系统(Source)

Human APLP2, His Tag (AP2-H52H9) is expressed from human 293 cells (HEK293). It contains AA Gly 32 - Ser 692 (Accession # Q06481-1).

Predicted N-terminus: Gly 32

Request for sequence

蛋白结构(Molecular Characterization)

Online(Gly 32 - Ser 692) Q06481-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 77.9 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human APLP2, His Tag (Cat. No. AP2-H52H9) SDS-PAGE gel

Human APLP2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

Human APLP2, His Tag (Cat. No. AP2-H52H9) MALS images

The purity of Human APLP2, His Tag (Cat. No. AP2-H52H9) is more than 90% and the molecular weight of this protein is around 80-105 kDa verified by SEC-MALS.

Report

背景(Background)

May play a role in the regulation of hemostasis. The soluble form may have inhibitory properties towards coagulation factors. May interact with cellular G-protein signaling pathways. May bind to the DNA 5'-GTCACATG-3'(CDEI box). Inhibits trypsin, chymotrypsin, plasmin, factor XIA and plasma and glandular kallikrein. Modulates the Cu/Zn nitric oxide-catalyzed autodegradation of GPC1 heparan sulfate side chains in fibroblasts.

 

 

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