膜杰作
Star Staining
分子别名（Synonym）
Delta3,delta-like 3 (Drosophila),delta-like protein 3,DLL3,Pudgy,SCDO1,SCDO1delta3
表达区间及表达系统（Source）
Human DLL3 (353-492) , His Tag (DL3-H52Ha) is expressed from human 293 cells (HEK293). It contains AA Arg 353 - Leu 492 (Accession # Q9NYJ7-1).
Predicted N-terminus: Arg 353
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 16.4 kDa. The protein migrates as 18 kDa and 19-21 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Supplied as 0.2 μm filtered solution in PBS, pH7.4.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
电泳（SDS-PAGE）
Human DLL3 (353-492) , His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
活性（Bioactivity）-ELISA
Immobilized Human DLL3 (353-492) , His Tag (Cat. No. DL3-H52Ha) at 1 μg/mL (100 μL/well) can bind Anti-DLL3 Antibody (specific Binding EGFs of DLL3), Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
背景（Background）
Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands.May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. DLL3 protein is expressed on the surface of tumor cells but not in normal adult tissues.
