膜杰作
Star Staining
分子别名（Synonym）
Glycoprotein B,gB,Envelope glycoprotein B
表达区间及表达系统（Source）
HCMV (strain Merlin) Glycoprotein B (gB), His Tag (CMB-V52H5) is expressed from human 293 cells (HEK293). It contains AA Ser 25 - Glu 698 (Accession # F5HB53-1 (I156H, H157R, W240N, Y242T, C246S, R457S, R460S)).
Predicted N-terminus: Ser 25
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 78.7 kDa. The protein migrates as 105-135 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
HCMV (strain Merlin) Glycoprotein B (gB), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of HCMV (strain Merlin) Glycoprotein B (gB), His Tag (Cat. No. CMB-V52H5) is more than 95% and the molecular weight of this protein is around 315-386 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized HCMV (strain Merlin) Glycoprotein B (gB), His Tag (Cat. No. CMB-V52H5) at 1 μg/mL (100 μL/well) can bind Anti-HCMV glycoprotein B antibody-SM5-1, Human IgG1 with a linear range of 0.2-8 ng/mL (QC tested).
Protocol
Immobilized Anti-HCMV glycoprotein B antibody-SM5-1, Human IgG1 at 1 μg/mL (100 μL/well) can bind HCMV (strain Merlin) Glycoprotein B (gB), His Tag (Cat. No. CMB-V52H5) with a linear range of 1-39 ng/mL (Routinely tested).
Protocol
背景（Background）
Human cytomegalovirus is a species of the Cytomegalovirus genus of viruses, which in turn is a member of the viral family known as Herpesviridae or herpesviruses. It is typically abbreviated as HCMV or, commonly but more ambiguously, as CMV. CMV Virus Envelope Glycoportein B (CMV-GB) can be cleaved into glycoprotein GP55. Envelope glycoprotein that plays a role in host cell entry, cell to-cell virus transmission, and fusion of infected cells. CMV-GB may be involved in the initial attachment via binding to heparan sulfate together with the gM/gN complex that binds heparin with higher affinity. Furthermore, CMV-GB can interact with host integrin ITGB1, PDGFRA and EGFR that likely serve as postattachment entry receptors. Also, CMV-GB participates in the fusion of viral and cellular membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL.
