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 >  Protein>GFR alpha-like >GFE-H5259

Human GFR alpha-like Protein, Fc Tag

分子别名(Synonym)

GFR alpha like,GFR alpha-like,GFRAL,GRAL

表达区间及表达系统(Source)

Human GFR alpha-like, Fc Tag (GFE-H5259) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Glu 351 (Accession # Q6UXV0-1 ).

Predicted N-terminus: Ser 19

Request for sequence

蛋白结构(Molecular Characterization)

GFR alpha-like Structure

This protein carries a human IgG1 Fc tag at the C-terminus

The protein has a calculated MW of 64.2 kDa. The protein migrates as 70-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

GFR alpha-like SDS-PAGE

Human GFR alpha-like, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

 

活性(Bioactivity)-ELISA

GFR alpha-like ELISA

Immobilized Human GDF-15, His Tag (Cat. No. GD5-H5149) at 1 μg/mL (100 μL/well) can bind Human GFR alpha-like, Fc Tag (Cat. No. GFE-H5259) with a linear range of 0.4-25 ng/mL (QC tested).

Protocol

 

背景(Background)

GFR alpha-like is also known as GDNF family receptor alpha-like, GFRAL, C6orf144, UNQ9356, PRO34128. Growth differentiation factor-15 (GDF15) is a circulating protein that has been implicated in multiple biological processes, including energy homeostasis, body weight regulation, and cachexia driven by cancer and chronic disease. GDNF family receptor α-like (GFRAL) was recently identified as the neuronal brainstem receptor responsible for mediating the anorectic actions of GDF15. Brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses . Upon interaction with its ligand, GDF15, interacts with RET and induces cellular signaling through activation of MAPK- and AKT- signaling pathways.

 

前沿进展

 
 
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GFR alpha-like靶点信息
英文全称:GDNF family receptor alpha-like
中文全称:
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:0详情
最高研发阶段:终止
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